(D) IgG eluted from excised nitrocellulose region corresponding to the ~ 90 kDa immunoreactive band yielded the ANNA3 immunostaining pattern when applied to mouse tissue sections; IgG eluted from the nitrocellulose region corresponding to ~170 kDa did not. (anti-Hu) and ANNA2 (anti-Ri) are recognized biomarkers of paraneoplastic autoimmunity and incorporated into serological evaluations of patients […]