Beads with LaP-1 immobilized in this manner were tested in isolations of RNA polymerase, Nup84, and Cdc33 as before. of overexpression, and high solubility after periplasmic purification. These crude periplasm preparations were incubated with PrA-Sepharose, and all constructs displayed high affinity, particular LaP-1, which was selected for follow-up studies (Supplementary Fig. 1A). In all cases, […]