Anti-V5 (Invitrogen) was used at 125 dilutions. Rbf1 through the entire amount of the proteins. The C-terminal half from the Rbf proteins display more general amino acidity similarity to individual pRb than p107 or p130. Hence, Rbf1 seems to have a divide character between pRb and p107. (C and D) Both domains from the Rbf1 […]