For macroscopic currents, KcsA was reconstituted in 1:100 (mass:mass) of protein to lipid ratio, while for single channel studies we used a ratio of 1 1:10,000 (mass:mass). at the selectivity Morphothiadin filter2. Recent high resolution crystallographic analyses have provided atomic level details of K+channels trapped with the activation gate in the closed3-5or open conformation6-9and the […]