Cells were transferred to experimental medium overnight, trypsinized, and cultured at a denseness of 10 104/250 l (main EVT) to 20 104/250 l (HTR-8/SVneo cells) in Matrigel-coated polycarbonate membrane invasion chamber inserts, which were immersed in cells tradition wells (24-well plates) containing 1% csFCS/RPMI (HTR-8/SVneo) or 0.2% csFCS-DMEM/F-12 (primary EVTs). transducer and activator of transcription […]