After purification, protein were lyophilized and aliquoted for storage space in 80C. == Alexa Fluor Labeling == Sixty nmol of V3C -synuclein were reacted with 300 nmol of Alexa Fluor 633 C5maleimide (Invitrogen, Carlsbad, CA) in 20 mM Tris, pH 7.5 for 2 h at room temperature. Areas, with over 60,000 fresh diagnoses every year […]